![]() افحص هذا WonderFox HD Video Converter Factory Pro 2021 تحميل مجاني MyEgy In contrast, insulin or FSH produced no effect on the ovarian explants. ![]() The addition of 1 U/ml of follicle stimulating hormone (FSH) resulted in maintenance of complete spermatogenesis. Addition of insulin $(5 \mu g/ml)$ to this medium produced striking effects on the testicular explants, particularly on the spermatogonial mitoses. ![]() After 3 wk of culture in hormone-free medium, germ cells showed a normal degeneration in testicular explants, whereas the ovarian explants retained normal histological characteristics as well as transcription and translation activities. ![]() Incubation was carried out at 20° C in a humidified atmosphere of 95:5% air and CO2. Testicular and ovarian fragments placed in "petriperm" dishes were cultured at pH 7.2 to 7.4 in bicarbonate buffered Medium 199 (M199), brought to osmolarity of 240 mOSM, supplemented with antibiotics. A method has been developed for culturing testes and ovaries of the young salamander Pleurodeles waltlii Michah. ![]()
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